Kim Annink

39 The pattern of Purkinje cell injury in neonates with HIE No significant differences were detected between the total number of PCs, i.e. normal, abnormal and non-classified PCs combined, in the anterior and posterior lobes (Table 3). For all analyzed locations, the total number of PCs was significantly higher at the crowns compared to the bases of the sulci (Figure 3). The percentage abnormal PCs was significantly higher at the bases of the sulci than in the crowns in the posterior lobules (p=0.011), as within the central folia (p=0.001) and peripheral folia (p=0.002) of the posterior lobules. In the anterior locations, the increase in abnormal PCs was only significant within the central folia of the anterior lobules (p=0.008). Table 3: Total PC count per 100 µm in anterior versus posterior lobes. Location Total PC count anterior vs. posterior p-value Anterior vs. posterior lobules All crowns 0.83 (0.72 – 1.01) vs. 1.29 (1.07 – 1.51) # 0.08 All bases of sulci 0.44 (0.27) vs. 0.48 (0.26) * 0.33 Central folia of anterior vs. posterior lobules All crowns 1.08 (0.24) vs. 0.86 (0.31) * 0.14 All bases of sulci 0.46 (0.18) vs. 0.43 (0.22) * ≈1.00 Peripheral folia of anterior vs. posterior lobules All crowns 0.99 (0.27) vs. 1.11 (0.22) * 0.68 All bases of sulci 0.56 (0.17) vs. 0.56 (0.20) * ≈1.00 The difference in total PC count per 100 µm between lobules II, III, V (anterior) and lobules VI, VIII, IX (posterior) of the vermis are presented. The paired samples t-test for normally distributed data and a Wilcoxon-signed rank test for non-Gaussian data was used. The crowns and bases of the sulci of the proximal and distal folia anterior/posterior are taken together. # Median (IQR), *Mean (SD). 2