Koos Boeve

151 Methylation biomarkers for the detection of tumor DNA in saliva Table 2. The sequences of the primers and probes for all genes used for methylation detection by QMSP and MSP Gene name Method QMSP forward 5’-3’ QMSP reverse 5’-3’ QMSP probe 6-FAM 5’-3’TAMRA Amplicon length T annealing Reference ACTB QMSP TGGTGATGGAGGAGGTTTAGTAAGT AACCAATAAAACCTACTCCTCCCTTAA ACCACCACCCAACACACAATAACAAACACA 133 60 NA C11orf85 QMSP GAAATGCGTACGCGTAGATC CAACTTCGAAACTCGTACCG TGGGAAGCGTATTTGCGCGTGC 118 60 NA, MethylCap-Seq EDNRB QMSP GGGAGTTGTAGTTTAGTTAGTTAGGGAGTAG CCCGCGATTAAACTCGAAAA TTTTTATTCGTCGGGAGGAG 75 60 Demokan et al.[19] HOXA9 QMSP AATAAATTTTATCGTAGAGCGGTAC CATATAACAACTTAATAACACCGAA GCGCCCCCATTAACCGTACGCGT 226 60 Guerrero-Preston et al.[24] NID2 QMSP GCGGTTTTTAAGGAGTTTTATTTTC CTACGAAATTCCCTTTACGCT ACGCCGCTACCCCAAACCTTACGA 99 62 Guerrero-Preston et al.[24] KCNA5 QMSP TTTTTTGACGTTAGGGTTAAGC GAACGCCTAACGTCAAACTC AGAGGGGTCGGTCGATCGTTGG 103 60 NA, MethylCap-Seq SIPA1 QMSP TTCGAGTCGAGGTTAGTTC CAAATCGACTAACCTCTTCG CGTAGCGGTAGCGATGTAGGC 124 60 NA, MethylCap-Seq TBX4 QMSP TTCGTTTTTAGTTCGAGTTGC CTACGCTCTCAATCCTACGC CGGCGTTAGTGGACGCGG 99 60 NA, MethylCap-Seq TIMP3 QMSP GCGTCGGAGGTTAAGGTTGTT CTCTCCAAAATTACCGTACGCG AACTCGCTCGCCCGCCGAA 95 62 Sun et al.(19) ACTB MSP TAGGGAGTATATAGGTTGGGGAAGTT AACACACAATAACAAACACAAATTCAC 103 57 Melchers et al.[29] DAPK meth MSP GGATAGTCGGATCGAGTTAACGTC CCCTCCCAAACGCCGA 98 60 Melchers et al.[29] DAPK unmeth MSP GGAGGATAGTTGGATTGAGTTAATGTT CCCTCCCAAACACCAACC 101 60 Melchers et al.[29] Abbreviations: NA, not applicable; (Q)MSP, (quantitative) methylation specific pcr; (un)meth, (un)methylated.