Koos Boeve

154 Chapter 8 Figure 2. DNA methylation levels of seven OSCC-specific markers in saliva cells of OSCC patients and healthy controls. QMSP analysis of seven methylation markers using DNA extracted from cells in saliva collected from 10 OSCC patients (saliva OSCC patients) and as healthy control saliva from five younger and five age-matched controls (saliva controls). Methylation levels on the x-axis are defined as the average DNA quantity of the gene of interest divided by the average DNA quantity of ACTB and then multiplied by 10,000. Dotted and continuous line represents median with interquartile range. Only statistically significant differences (p < 0.050, using the Mann-Whitney-U test) between saliva of 10 controls and 10 OSCC samples are shown.