Koos Boeve

167 Methylation biomarkers for the detection of tumor DNA in saliva Supplementary data 1. Continued Gene name Chromosome locus Gene regio start (GSE42409) Gene regio end (GSE42409) p-value (Mann- Whitney U test) Number of reads OSCC patient 1 OSCC patient 2 OSCC patient 3 OSCC patient 4 OSCC patient 5 OSCC patient 6 OSCC patient 7 OSCC patient 8 OSCC patient 9 OSCC patient 10 OSCC patient 11 OSCC patient 12 Leukocyte pool 1 Leukocyte pool 2 MLNR 13 49794460 49795110 0.042 11 3 12 5 6 6 8 19 7 11 6 5 5 3 FSIP2 2 186603232 186604189 0.043 3 9 7 7 6 5 11 11 9 4 13 4 4 2 FGF3 11 69633336 69634068 0.044 2 2 12 5 1 7 9 6 10 7 38 3 2 2 CLSTN3 12 7280735 7280996 0.044 6 10 9 9 11 7 11 11 11 8 17 5 15 13 DHODH 16 72041197 72041688 0.046 5 8 5 9 6 7 10 6 10 8 11 4 10 9 AC022400.1 10 75491360 75491675 0.046 10 15 10 12 7 5 19 12 10 16 17 6 9 8 KRT33A 17 39506596 39507113 0.046 8 13 6 4 10 8 15 9 8 3 14 8 11 12 AL512638.1 1 115826147 115826583 0.047 6 11 7 6 6 6 8 11 7 5 11 7 9 9 AC092810.2 1 209405064 209405472 0.047 5 16 5 7 7 7 8 15 5 4 13 5 3 5 TNNT3 11 1940716 1941338 0.048 9 10 10 6 5 9 6 12 4 10 12 7 14 12 HSF5 17 56565440 56565821 0.048 11 11 9 9 9 4 10 11 13 6 14 4 6 4 AP002748.2 11 66304685 66305454 0.048 6 11 5 7 4 10 10 5 8 9 16 5 6 5 FAM100A 16 4665443 4666047 0.048 3 11 9 15 5 7 6 9 11 12 13 6 12 11 EDARADD 1 236511487 236512106 0.049 4 13 12 7 6 8 18 16 11 7 14 5 13 13 FAM21C 10 46220649 46221042 0.050 3 5 6 9 5 11 8 9 13 8 10 5 11 13 Abbreviations: Chr, chromosome; TSS, transcription start side; FDR, false discovery rate; bp, base pair). Location of the methylation as extracted from the “Map of the Human Methylome” [http://www.biobix.be/map-of-the-human- methylome/, BIOBIX (Lab of Bioinformatics and Computational Genomics), Ghent, University of Ghent, Belgium 2014]