Koos Boeve

206 Chapter 10 Quantitativemethylation specific PCR analysis using saliva fromtenOSCCpatients compared to ten non-cancer controls of similar or younger age, revealed only a difference for KCNA5 methylation. Moreover, when KCNA5 was combined with other markers, the combination with TIMP3 revealed a 100% diagnostic potential in detecting OSCC patients compared to non-cancer controls of similar age using saliva. We concluded that this newly discovered KCNA5 by MethylCap-Seq combined with TIMP3 might be useful for early detection of local recurrence or second primary tumours in saliva cells of OSCC patients.