Peter van Mourik

114 Chapter 4 Table 4.Dissemination of data processing: from raw data to area under the curve (as applied and programmed in Excel). Total organoid area µm 2 (per time point, per well) Calculations Well nr 1 2 3 4 5 6 7 8 Time point (min) 0.02 µM fsk (duplo) 0.128 µM fsk (duplo) 0.8 µM fsk (duplo) 5 µM fsk (duplo) 0 (Raw data) 1 281330 238453 263766 188102 331433 321666 300166 314690 10 2 283573 239998 263915 189347 345534 342520 388137 413799 20 3 284395 241493 270094 194081 381535 382332 476159 510839 30 4 283523 240770 274130 201630 416141 427078 546616 590889 40 5 283548 243810 285890 207609 455630 463079 604865 654868 50 6 287061 243062 294186 215582 490858 499504 653024 705319 60 7 286687 241543 303803 223131 517691 531742 684018 750688 STEP A Normalized to time point 0 per well (baseline set to 100%) (1/1) x 100 8 100,00 100,00 100,00 100,00 100,00 100,00 100,00 100,00 (2/1) x 100 9 100,80 100,65 100,06 100,66 104,25 106,48 129,31 131,49 (3/1) x 100 10 101,09 101,27 102,40 103,18 115,12 118,86 158,63 162,33 (4/1) x 100 11 100,78 100,97 103,93 107,19 125,56 132,77 182,10 187,77 (5/1) x 100 12 100,79 102,25 108,39 110,37 137,47 143,96 201,51 208,10 (6/1) x 100 13 102,04 101,93 111,53 114,61 148,10 155,29 217,55 224,13 (7/1) x 100 14 101,90 101,30 115,18 118,62 156,20 165,31 227,88 238,55 STEP B Absolute baseline area 8 -100 15 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 9 -100 16 0,80 0,65 0,06 0,66 4,25 6,48 29,31 31,49 10 -100 17 1,09 1,27 2,40 3,18 15,12 18,86 58,63 62,33