Peter van Mourik

115 Forskolin-induced swelling protocol Table 4.Dissemination of data processing: from raw data to area under the curve (as applied and programmed in Excel). Total organoid area µm 2 (per time point, per well) Calculations Well nr 1 2 3 4 5 6 7 8 Time point (min) 0.02 µM fsk (duplo) 0.128 µM fsk (duplo) 0.8 µM fsk (duplo) 5 µM fsk (duplo) 11 -100 18 0,78 0,97 3,93 7,19 25,56 32,77 82,10 87,77 12 -100 19 0,79 2,25 8,39 10,37 37,47 43,96 101,51 108,10 13 -100 20 2,04 1,93 11,53 14,61 48,10 55,29 117,55 124,13 14 -100 21 1,90 1,30 15,18 18,62 56,20 65,31 127,88 138,55 STEP C Area difference between time points 16-15 22 0,80 0,65 0,06 0,66 4,25 6,48 29,31 31,49 17-16 23 0,29 0,63 2,34 2,52 10,86 12,38 29,32 30,84 18-17 24 -0,31 -0,30 1,53 4,01 10,44 13,91 23,47 25,44 19-18 25 0,01 1,27 4,46 3,18 11,91 11,19 19,41 20,33 20-19 26 1,25 -0,31 3,15 4,24 10,63 11,32 16,04 16,03 21-20 27 -0,13 -0,64 3,65 4,01 8,10 10,02 10,33 14,42 STEP D Surface area per 10 minute interval 16 x10/2 28 3,99 3,24 0,28 3,31 21,27 32,42 146,54 157,47 17 x 10 -( 23 x 10/2) 29 9,43 9,61 12,28 19,20 96,86 126,72 439,70 469,13 18 x 10 - ( 24 x 10/2) 30 9,34 11,23 31,64 51,85 203,37 258,15 703,68 750,50 19 x 10 - ( 25 x 10/2) 31 7,84 16,09 61,58 87,81 315,15 383,67 918,07 979,34 20 x 10 - ( 26 x 10/2) 32 14,13 20,90 99,60 124,90 427,87 496,25 1095,32 1161,15 21 x 10 - ( 27 x 10/2) 33 19,71 16,14 133,56 166,16 521,50 602,98 1227,17 1313,40 STEP E Area under the curve (AUC) Total area Sum of 28 - 33 Y 64,4 77,2 339 453,2 1586 1900,2 4530,5 4831 4