Peter van Mourik

117 Forskolin-induced swelling protocol REFERENCES Boj, S. F., Vonk, A. M., Statia, M., Su, J., Vries, R. R. G., Beekman, J. M., & Clevers, H. (2017). Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients. Journal of Visualized Experiments , (120), 1–12. https://doi.org/10.3791/55159 Berkers G., van Mourik P. Rectal Organoids Enable Personalized Treatment of Cystic Fibrosis. Cell Rep.;26(7):1701-1708.(2019) Dekkers, J. F., Berkers, G., Kruisselbrink, E., Vonk, A., de Jonge, H. R., Janssens, H. M., … Beekman, J. M. (2016). Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis. Science Translational Medicine , 8 (344), 344ra84. https://doi.org/10.1126/scitranslmed. aad8278 Dekkers, J. F., Wiegerinck, C. L., de Jonge, H. R., Bronsveld, I., Janssens, H. M., de Winter-de Groot, K. M., … Beekman, J. M. (2013). A functional CFTR assay using primary cystic fibrosis intestinal organoids. Nature Medicine , 19 (7), 939– 945. https://doi.org/10.1038/nm.3201 Servidoni, M. F., Sousa, M., Vinagre, A. M., Cardoso, S. R., Ribeiro, M. A., Meirelles, L. R., … Amaral, M. D. (2013). Rectal forceps biopsy procedure in cystic fibrosis: technical aspects and patients perspective for clinical trials feasibility. BMC Gastroenterology , 13 , 91. https://doi.org/10.1186/1471-230X-13-91 McQuin C, Goodman A, Chernyshev V, Kamentsky L, Cimini BA, Karhohs KW, …, Carpenter AE (2018). CellProfiler 3.0: Next-generation image processing for biology. PLoS Biol. 16(7):e2005970 / doi. PMID: 29969450 4