Peter van Mourik

123 Centralized intestinal organoid generation RESULTS Table 1. Subject characteristics and outcomes Total population Mean +/- SD (range) or n (%) Rectal suction biopsy Mean +/- SD (range) or n (%) Forceps biopsy Mean +/- SD (range) or n (%) Number of patients (%) 502 222 (54.8) 280 (55.6) Age (years, mean) 34 +/- 11.5 (16 – 77) 32.0 +/- 10.7 (16 – 67) 32.9 +/- 12.1 (16 – 77) Sex (female) 257 (51.2) 123 (55.4) 134 (47.9) Biopsies per patient 3.6 +/- 1.3 (1 – 8) 3.6 +/- 1.4 (1 – 8) 3.7 +/- 1.2 (1 – 7) Adverse events 2 (0.4) 2 (0.8) 0 Serious adverse events 2 (0.4) 2 (0.8) 0 Gastroenteritis 1 1 (0.4) 0 Hemorrhage 1 1 (0.2) 0 Successful organoid generation 477 (95.0) 214 (96.4) 262 (93.6) Reasons for failure Insufficient sample viability 16 (3.2) 3 (1.4) 13 (4.6) Contamination of sample 8 (1.6) 4 (1.8) 4 (1.4) Shipment error 1 (0.2) 1 (0.5) 0 502 adult subjects from 33 different hospitals participated in the study. Gastroenterology departments at all sites were able to perform either forceps biopsy or rectal suction biopsy procedures. Forceps biopsies were performed at 28 sites in 280 subjects, while rectal suction biopsies were performed at 9 different sites in 222 subjects. Four sites performed both procedures. Two adverse events were reported for the rectal suction biopsy procedures, which were considered serious adverse events. One biopsy procedure led to rectal bleeding which had to be surgically resolved and another associated with campylobacter gastroenteritis, which was probably unrelated to the procedure. No other (serious) adverse events were reported. No other (serious) adverse events were reported. 5