Peter van Mourik

195 General discussion intestinal organoids with other pre-clinical models such as HBEs and HNEs should further elucidate how CFTR-function in intestinal organoids relates to respiratory disease severity. Future clinical studies should elucidate thresholds of organoid swelling that can predict whether a patient will or will not respond to a specific drug (combination), and whether these thresholds are identical for drugs with different modes-of-action, or whether drug-specific thresholds are necessary. Moreover, concerted efforts should ensure that widely available organoid biobanks are created that enable access to a diverse range of (intestinal) tissues to further expand our knowledge of CF and CFTR-directed therapeutics. 9