Peter van Mourik

33 R117H function and VX-770 response in organoids Supplementary table 2. Primer sequences and amplicon sizes. Gene Forward primer sequence (5’ -> 3 ’) Reverse primer sequence (5’ -> 3’) Amplicon size (bp) Source ACTB CTGGAACGGTGAAGGTGACA AAGGGACTTCCTGTAACAATGCA 117 [a] GAPDH TGCACCACCAACTGCTTAGC GGCATGGACTGTGGTCATGAG 87 [b] B2M CTCCGTGGCCTTAGCTGTG TTTGGAGTACGCTGGATAGCCT 69 [c] GUSB GAAAATATGTGGTTGGAGAGCTCATT CCGAGTGAAGATCCCCTTTTTA 101 [d] HMBS CACGATCCCGAGACTCTGCT TACTGGCACACTGCAGCCTC 81 [d] HPRT1 TGAGGATTTGGAAAGGGTGT GAGCACACAGAGGGCTACAA 118 [d] TBP GAGAGTTCTGGGATTGTACCG ATCCTCATGATTACCGCAGC 143 [e] SDHA TGGTTGTCTTTGGTCGGG GCGTTTGGTTTAATTGGAGGG 85 [e] YWHAZ ATGCAACCAACACATCCTATC GCATTATTAGCGTGCTGTCTT 178 [e] RPS13 CGAAAGCATCTTGAGAGGAACA TCGAGCCAAACGGTGAATC 87 [e] R117H-CFTR ATTTAGGGGAAGTCACCAAAGCAG GCCTAGATAAATCGCGATAGATT 107 [f] CFTR ATTTAGGGGAAGTCACCAAAGCAG GCCTAGATAAATCGCGATAGAGC 107 [f] [a] RtprimerDB: http://www.rtprimerdb.org/ [b] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002 Jun 18;3(7) [c] Cicinnati VR, Shen Q, Sotiropoulos GC, Radtke A, Gerken G, Beckebaum S. Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR. BMC Cancer. 2008 Nov 27;8:350. [d] Valente V, Teixeira SA, Neder L, Okamoto OK, Oba-Shinjo SM, Marie SKN, et al. Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR. BMC Mol Biol. 2009;10:17. [e] Liu L-L, Zhao H, Ma T-F, Ge F, Chen C-S, Zhang Y-P. Identification of valid reference genes for the normalization of RT-qPCR expression studies in human breast cancer cell lines treated with and without transient transfection. PloS One. 2015;10(1):e0117058. [f] Developed in this study using primer-BLAST: https://www.ncbi.nlm.nih.gov/tools/primer-blast/ and guidelines published by Wangkumhang et al. 6 . 2