Peter van Mourik

37 R117H function and VX-770 response in organoids REFERENCES 1. Dekkers, J. F. et al. A functional CFTR assay using primary cystic fibrosis intestinal organoids. Nat. Med. 19 , 939–45 (2013). 2. Dekkers, J. F. et al. Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis. Sci. Transl. Med. 8 , 344ra84 (2016). 3. Boj, S. F. et al. Forskolin-induced Swelling in Intestinal Organoids: An <em>In Vitro</em> Assay for Assessing Drug Response in Cystic Fibrosis Patients. J. Vis. Exp. 1–12 (2017) doi:10.3791/55159. 4. Hellemans, J. & Vandesompele, J. Selection of reliable reference genes for RT-qPCR analysis. Methods Mol. Biol. 1160 , 19–26 (2014). 5. Vandesompele, J. et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3 , (2002). 6. Wangkumhang, P. et al. WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations. BMC Genomics 8 , 275 (2007). 2