Peter van Mourik

74 Chapter 4 REAGENT or RESOURCE SOURCE IDENTIFIER Y-27632 Dihydrochloride (ROCKi) Abmole bioscience #Y-27632 Zeocin (100 mg/mL) 1g Thermo Fisher Scientific: Invitrogen #R25001 Experimental Models: Cell Lines HEK293T – Noggin hFc cell line Optimal Noggin-hFc producing subclone obtainable through the HUB: http://hub4organoids.eu/ n/a L- Wnt 3A producing cell line ATCC Optimal Wnt-3A producing subclone obtainable through the HUB: http:// hub4organoids.eu/ #CRL-2647 Human colon organoids genotype G542X / G542X HUB: http://hub4organoids.eu/ #HUB- 02-D2-121 Human colon organoids genotype F508del / R1162X HUB: http://hub4organoids.eu/ #HUB- 02-D2-038 Human colon organoids genotype F508del/ F508del HUB: http://hub4organoids.eu/ #HUB- 02-D2-341 Human colon organoids genotype F508del/ G551D HUB: http://hub4organoids.eu/ #HUB- 02-D2-043 Human colon organoids genotype F508del/S1251N HUB: http://hub4organoids.eu/ #HUB- 02-D2-103 Human colon organoids genotype F508del / R117H - 7T HUB: http://hub4organoids.eu/ #HUB- 02-D2-004 Software and Algorithms Cell profiler (open- source) McQuin et al., 2018 https:// cellprofiler.org/ Zen blue® Zeiss https://www. zeiss.com/ microscopy/ int/products/ microscope- software/zen. html Graphpad Prism Graphpad https://www. graphpad. com/scientific- software/ prism/ Microsoft Excel Microsoft Corporation https://office. microsoft.com/ excel Other 15 and 50 mL conical tubes No recommended vendor n/a Microfuge tubes No recommended vendor n/a