Peter van Mourik

75 Forskolin-induced swelling protocol REAGENT or RESOURCE SOURCE IDENTIFIER 100 mL (plastic) bottles No recommended vendor n/a 0.22 µm filters No recommended vendor n/a Serological pipets No recommended vendor n/a Micropipet filter tips + Micropipet tips without filter (200 µL) No recommended vendor n/a Cryovials No recommended vendor n/a 24-wells flat bottom tissue culture plates / 96-wells flat bottom tissue culture plates No recommended vendor n/a T175 cell culture flasks No recommended vendor n/a 145 mm petridishes No recommended vendor n/a Cell Freezing Container (e.g. Mr Frosty) No recommended vendor n/a Light/optical microscope appropriate for cell culture with standard ocular (10x) and 4x and 10x objective No recommended vendor n/a Live cell imaging confocal microscope with 5x objective, incorporated incubator (allowing controlling temperature and CO2) and 488 nm laser Recommended but not essential: Zeiss LSM800 n/a Liquid nitrogen tank No recommended vendor n/a Note: clinical reagents and materials mentioned in ‘obtaining human (colon) rectal biopsies’ (e.g. rectal suction biopsy device, forceps device, video endoscope) are not included in this list and are often hospital-specific. For information please contact your local gastroenterologist. 4