Peter van Mourik

77 Forskolin-induced swelling protocol Stock preparation of human colon organoid medium components Timing ~3 h Human colon organoid medium consists of several chemical components as well as self-produced conditioned media, such as Wnt-3A conditioned media (WCM) and Noggin conditioned media (NCM). In this protocol hR-spondin-3 (R-spo3) was preferred over self-produced R-spondin-1 conditioned media. However, a R-spondin-1 producing cell line is available and could be preferred due to cost reduction of media. 4