Peter van Mourik

81 Forskolin-induced swelling protocol A. Forceps biopsy procedure: Additional information on forceps biopsy procedure including video can be found elsewhere (Servidoni et al., 2013). Note: A nurse/endoscopy-assistant guides the patient through the process and assists the physician throughout the procedure. CAUTION: The (jumbo) forceps should be closed and pulled back when entering or exiting the endoscopic instrumentation channel. CAUTION: The procedure should not take place in case there are visibility problems during the endoscopy. Note: Before starting the procedure, be sure you have read and understood all the instructions detailed in this paragraph. 1. Make sure the patient agrees to the procedure before starting and the appropriate informed consent form is signed. 2. The nurse opens the 50 mL collection tube containing PBS0 or storage media (Ad+++ with additional gentamicin (50 µg/mL) and vancomycin (50 µg/mL)) at 4°C and properly labelled for the rectal biopsies (with clean gloves) . 3. The nurse proceeds by opening a new sterile jumbo forceps packaging, removing the cover at the end of the forceps around the mouth of it and presents it to the physician. 4. The physician enters the forceps inside the endoscopic instrumentation channel. 5. The nurse/assistant opens or closes the forceps as instructed by the physician. 6. When removing the forceps out of the endoscopic instrumentation channel it should be cleaned with a gauze to avoid blood spatters. The biopsies are collected in the collection tube. Note: If the rectal/ colon biopsy is sticking to the jumbo forceps, use a sterile needle or sterile tweezers to remove the biopsy. 7. Rinse the forceps in a cup of clean water before re-entering the jumbo forceps into the endoscope to collect more biopsies from the patient. Make sure the forceps is clean. 4