Peter van Mourik

83 Forskolin-induced swelling protocol B. Obtaining biopsies by rectal suction device A nurse/endoscopy-assistant guides the patient through the process and assists the physician throughout the procedure. Note: Before starting the procedure, make sure to have read and understood all the instructions detailed in this paragraph Figure 1. Rectal suction biopsy device with manometer for defined suction pressure and syringe to create suction. 1. Make sure the patient agrees to the procedure before starting and the appropriate informed consent form is signed. 2. Ensure an appropriate bowel preparation: apply an enema to the patient (Sodium phosphate enema (2.5 mL* (per) kg body weight; maximum dose 130 mL) by inserting the sodium phosphate solution rectally +/- 2-3 h before the biopsy procedure. 3. Wait for the result of the enema. 4. Test the rectal suction device for vacuum pressure before using it in the patient (see figure 1). 4