Peter van Mourik

95 Forskolin-induced swelling protocol Figure 10. Examples of 7 day old human colon organoid cultures (bright field, 4 x magnification). A. Typical pre-swollen stem cell phenotype of a high quality, proliferated wild type (non-CF) human colon organoid culture. B. Non-swollen stem cell phenotype of a high quality, proliferated CF colon organoid culture. C. Example of a bad quality CF colon organoid culture with thick-walled, differentiated structures with decreased stem cell phenotype which is often due to low activity of Wnt-3A in WCM. 4