Ingrid 't Hart

27 Chemoenzyma�c synthesis of DSGb5 2 Chapter 2 Chemoenzyma�c synthesis of the oligosaccharide moiety of the tumor-associated an�gen disialosyl globopentaosylceramide Abstract : Disialosyl globopentaosylceramide (DSGb5) is o�en expressed by renal cell carcino- mas. To inves�gate proper�es of DSGb5, we have prepared its oligosaccharide moiety by chem- ically synthesizing Gb5 which was enzyma�cally sialylated using the mammalian sialyltrans- ferases ST3Gal1 and ST6GalNAc5. Glycan microarray binding studies indicate that Siglec-7 does not recognize DSGb5, and preferen�ally binds Neu5Acα(2,8)Neu5Ac containing glycans. Ingrid M.E. ‘t Hart, Tiehai Li, Margreet A. Wolfert, Shuo Wang, Kelley W. Moremen, and Geert-Jan Boons Adapted from: Org. Biomol. Chem. , 2019, 17, 7304