Fehmi Keçe

Chapter 3 92 3.7. Supplemental Methods 3.7.1 Laboratory Measurements Intrinsic and extrinsic coagulation APTT and PT/INR were respectively measured on an automated coagulometer using STA Neoplastin R (STA-R Max, Diagnostica Stago, Leiden, Netherlands) and using STA Cephascreen (STA-R Max, Diagnostica Stago, Leiden, Netherlands). Fibrin turnover For the measurement of D-dimer an automated coagulometer using STA Liatest D-DI Plus (STA-R Max, Diagnostica Stago, Leiden, Netherlands) was used. Coagulant potential (acute phase marker) Fibrinogen was measured with STA Liquid Fib (STA-R Max, Diagnostica Stago, Leiden, Netherlands). Endothelial damage For the measurement of Von Willebrand Factor Antigen, ELISA using rabbit polyclonal anti-human VWF antibody (A082, Dako, Denmark) and horseradish peroxidase conjugated rabbit anti-human VWF antibody (P0226, Dako, Denmark) was used. Thrombin generation Prothrombin Fragment 1+2 was measured with ELISA (Cloud-Clone Corp., Katy, TX, USA). Fibrinolysis Tissue plasminogen activator was measured using Elisa (Abcam, Cambridge, United Kingdom). Platelet activation Soluble P-selectin (sP-sel) was measured on ELISA (Affymetrix, part of the Thermo Fisher Scientific, Waltham, MS, USA).