Feline Lindhout

3 Centrosome-mediated microtubule remodeling during axon formation in human iPSC-derived neurons 101 Supplementary Figure 1. Centrosomes display MTOC function and local Trim46 appearance during axon specification A. Typical examples of human iPSC-derived NSCs (day 0), HeLa cells, primary dissociated rat neurons (day 0) and mouse IMCD3 cells immunostained for Trim46 and Centrin, and co-immunos- tained with Nestin or α-Tubulin if indicated. Inserts represent centrosomes. Scale bars: 10µm in overview, 2 µm in inserts. B. Typical examples of mature human iPSC-derived glia cells (day 17) immunostained for Trim46, Centrin and α-Tubulin. Zooms represent centrosomes. Scale bars: 20 µm in overview, 1 µm in zooms