Feline Lindhout

VAP–SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function 145 4 Supplementary Figure 3. VAP-SCRN1 interaction at the ER is mediated by a single FFAT-like motif A. Schematic representation of the four potential FFAT-like motifs in SCRN1. Amino acid position number 2 (orange) of the FFAT-like motifs was mutated to alanine residues. B. Quantifications of SCRN1 recruitment to VAPA-positive structures in COS7 cells (%) co-expressing HA-VAPA with GFP or GFP-SCRN1, GFP-SCRN1-N, GFP-SCRN1-C, GFP-SCRN1-Y40A, GFP- SCRN1-F144A, GFP-SCRN1-F153A, or GFP-SCRN1-F402A. N = 2–3, n = 39–59. C. COS7 cells co-expressing HA-VAPA with GFP-SCRN1-Y40A, GFP-SCRN1-F144A, GFP-SCRN1- F153A, or GFP-SCRN1-F402A. Scale bars: 10 μm (full size) and 5 μm (zoom). D. Hippocampal neurons (DIV16) co-expressing HA-VAPA with GFP-SCRN1-Y40A, GFP-SCRN1- F144A, GFP-SCRN1-F153A, or GFP-SCRN1-F402A. Scale bars: 10 μm (full size) and 5 μm (zoom). Data information: ***P < 0.001, by chi-square test with post hoc analysis including Bonferroni correction.