Feline Lindhout

Quantitative mapping of transcriptome and proteome dynamics during polarization of human iPSC-derived neurons 2 67 Supplementary Figure 3. Extra developmental stage and gradual action potential maturation during axon specification A. Quantifications of distance soma to distal end of Trim46 signal in neurites of stage 2b, 3a and 3b human iPSC-derived neurons. N=2, n=20 cells. B. Quantifications of total AnkG length in neurites of stage 2b, 3a and 3b human iPSC-derived neurons. N=2, n=14-20 cells. C. Quantifications of distance soma to start of the AnkG signal in neurites of stage 2b, 3a and 3b human iPSC-derived neurons. N=2, n=14-20 cells. D. Quantifications of distance soma to distal end of AnkG signal in neurites of stage 2b, 3a and 3b human iPSC-derived neurons. N=2, n=14-20 cells. E. Representative images of localization of voltage-gated sodium channels in stage 3a and 3b human iPSC-derived neurons. Cells were immunostained for Trim46, AnkG, PanNaV and β3-tubulin. Arrowheads mark PanNaV localizations. Scale bar: 20 µm in overview, 5 µm in zoom. Used tests: One-way ANOVA including post-hoc analysis with Bonferroni correction ( A - D ); *** p<0.001, ** p<0.01, * p<0.05, ns p≥0.05; error bars are ± SEM.