Feline Lindhout

3 94 Control human iPSC sample, day 7 = TMT-128 / Centrinone-B human iPSC sample, day 7 = TMT-131 Following incubation at room temperature for 1 hour, the reaction was quenched using 5% hydroxylamine. Differentially TMT-labeled peptides were mixed in equal ratios and dried in a vacuum concentrator. Peptide fractionation, MS analysis, data processing, and bioinformatic analysis of the samples were performed as described before (Lindhout et al. 2020).The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD020227 (Perez-Riverol et al. 2019). Statistical analysis All statistical analyses were performed using Prism GraphPad (version 8.0) software. Statistical tests are described in the corresponding Figure legends. P-values are annotated as follows: * p<0.05, ** p<0.01, *** p<0.001, and ns p≥0.05.