Anne-Marie Koop

4 163 Figure 1 . Hemodynamic and molecular changes over time in RV pressure load. PAB-gradient measured by Doppler echocardiography (A). Right ventricular systolic and diastolic function expressed by TAPSE (B) and dP/dt Max corrected for RVpeakpressure (C), and dP/dt Min corrected for RV peakpressure (D) and RV EDP (E). Cardiac index (cardiac output corrected for bodyweight) (F). Power (RV peakpressure multiplied by stroke volume) (G). RV weight correct for bodyweight (H), RV cross sectional area (I), RV capillary myocyte ratio (K) and RV fibrosis (L). Representative images of histological analyses: wheat germ agglutinin, lectin and masson-trichrome staining respectively (ruler is 50 µm) (J). mRNA expression of genes involved in cardiomyocyte stress, hypertrophy, fetal gene program, and fibrosis (M). Data presented as mean±SEM. * = p < 0.05 compared to control, # = p < 0.05 compared to indicated time point. ● = individual animal, white = control group, grey = pulmonary artery banding groups. PAB = pulmonary artery banding. 2w, 5w and 12w = 2, 5 and 12 weeks after PAB. TAPSE = tricuspid annular plane systolic excursion, dPdt = delta pressure delta time, EDP = end diastolic pressure. . CCSA = cross cardiomyocyte sectional area. NPPA = natriuretic pro- peptide type A, RCAN1 = regulator of calcineurin 1, MHC = myocyte heavy chain, COL1A2 = collagen subunit 1A2, COL3A1 = collagen subunit 3A1, TGF- β = transforming growth factor β .