Anne-Marie Koop

164 pyruvate nmol O 2 /min/mg protein 2w 5w 12w 0 500 1000 # palmitoyl CoA nmol O 2 /min/mg protein 2w 5w 12w 0 100 200 300 400 500 pyruvate/palmitoyl CoA ratio 2w 5w 12w 0 1 2 3 4 5 * * A B C max. respiratory capacity citrate synthase μmol/mg/min control 2w 5w 12w 0 20 40 60 MCAD control 2w 5w 12w 0.0 0.5 1.0 1.5 * mRNA level (fold change) CPT1B control 2w 5w 12w 0.0 0.5 1.0 1.5 2.0 2.5 mRNA level (fold change) GLUT4 control 2w 5w 12w 0.0 0.5 1.0 1.5 2.0 2.5 * * * mRNA level (fold change) Figure 2 n = 3-13 in each group * Figure 2 . Right ventricular fatty acid and glucose metabolism during pressure load over time. Maximal mitochondrial respiratory capacity (state 3 uncoupled) measured by oxygen consumption rates in isolated mitochondria from RV tissue in the presence of palmitoyl coA or pyruvate, and the ratio of these two (A). Citrate synthase as marker of mitochondrial content (B). mRNA level of regulatory genes in metabolic transport and regulation (C). Data presented as mean ± SEM. * = p < 0.05 compared to control, # = p < 0.05 compared to indicated time point. ●  = individual animal, white = control group, grey = pulmonary artery banding groups, 2w, 5w and 12 w = 2, 5, and 12 weeks. O2 = oxygen. MCAD = medium-chain acyl coA, PPARα  = peroxisome proliferator-activated receptor a, PGC1α  = PPAR gamma coactivator gene 1α , CPT1B = carnitine palmitoyltarnsferase isoform 1B, GLUT4 = glucose transporter 4.