Anne-Marie Koop

4 167 non-phospholipids phospholipids precursor phospholidpids relative abundance control PAB A E F D CL(72:8) vs . s um of other cardiolipins relative abundance CL(72:8) sum other cardiolipins 500 1000 1500 2000 2500 6000 8000 10000 12000 14000 G heatmap non-phospholipids heatmap cardiolipins B C * q=0.047 TG(54:4) TG(57:4) TG(58:3) TG(59:4) TG(60:3) TG(60:4) TG(60:5) TG(61:4) TG(62:4) TG(62:5) DG(36:4) DG(36:5) DG(37:5) DG(38:1) DG(38:2) DG(38:5) DG(40:3) DG(40:4) DG(42:5) DG(42:6) Cer(d34:0) Cer(d36:0) Cer(d39:1) Cer(d39:2) Cer(d40:1) Cer(d40:2) Cer(d41:2) Cer(d41:3) CL(68:3) CL(68:4) CL(68:5) CL(68:8) CL(70:4) CL(71:7) CL(74:10) CL(72:6) CL(72:8) CL(72:9) CL(74:11) CL(74:8) CL(76:11) CL(76:12) CL(78:14) relative abundance relative abundance triglyceride 62 cluster log 10 value 62:0 62:1 62:2 62:3 62:4 62:5 62:6 62:7 62:8 62:9 62:1062:11 62:12 62:13 62:14 0.01 0.1 1 10 diglyceride 42 cluster 42:0 42:1 42:2 42:4 42:5 42:6 42:7 42:8 42:9 42:10 0.001 0.01 0.1 1 10 100 log 10 value * * * * * * * * * * * Figure 3 CON_A CON_B CON_C CON_D CON_E PAB_A PAB_B PAB_C PAB_D PAB_E CON_A CON_B CON_C CON_D CON_E PAB_A PAB_B PAB_C PAB_D PAB_E n = 5 in each group TG DG Cer 0 1 2 3 4 5 5000 10000 15000 * CL PC PE 5000 10000 15000 20000 * PI PG PA 0 200 400 600 2000 2500 3000 3500 Figure 3 . Right ventricular lipid content. Class totals of non-phosholipids (A), phospholipids (B), and precursor phospholipids (C). Heatmap of individual significantly changed non-phospholipids selected on top ten VIP- score within class (D). Heatmap of individual cardiolipins VIP > 1 (E). CL(72:8) versus sum of other cardiolipin (F). Representive examples of progressieve decrease of poly-unsaturated fatty acids within lipid clusters of TG and DG (G). TG = triglyceride, DG = diglyceride, Cer = ceramide, PC = phosphatidylcholine, PE = phosphatidylethanolamine, PI = phosphatidylinositol, PG = phosphatidylglycerol, PA = phosphatidic acid, CL = cardiolipin .