Anne-Marie Koop

182 Supplemental table. Sequence of primers used for quantitative real-time PCR sequence forward primer sequence reverse primer GAPDH TCTCTGCTCCTCCCTGTTCTA TACGGCCAAATCCGTTCACA α MHC GACAACTCCTCCCGCTTTGG AAGATCACCCGGGACTTCTC β MHC GTCAAGCTCCTAAGTAATCTGTT GAAAGGATGAGCCTTTCTTTGC RCAN1 TTAAGCGTCTGCCCGTTGAA CCTGGTCTCACTTTCGCTGA NPPA ATGGGCTCCTTCTCCATCAC TCTACCGGCATCTTCTCCTC COL1A2 ATGGTGGCAGCCAGTTTG GCTGTTCTTGCAGTGGTAGG COL3A1 AGAGGATGGCTGCACTAAAC CTTGATCAGGACCACCAATG TGF β 1 AAGAAGTCACCCGCGTGCTA TGTGTGATGTCTTTGGTTTTGTCA TGF β 2 AAATCGACATGCCGTCCCAC GGATGGCATCAAGGTACCCAC MCAD CCGTTCCCTCTCATCAAAAG ACACCCATACGCCAACTCTT PPAR α ATGAGTCCCCTGGCAATG GGCATTCTTCCAAAACGG PGC1 α ACCGTAAATCTGCGGGATGATG CATTCTCAAGAGCAGCGAAAGC CD36 TGCAAAGAAGGAAAGCCTGTG GCTCATCTTCGTTAGGATTCAAGC CPT1b TTCCTGGACGAGGTGCTTTC TTGGGGTACTGCTTTGGGTC GLUT1 GCTGTGGCTGGCTTCTCTAA CCGGAAGCGATCTCATCGAA GLUT4 CCGTGGCCTCCTATGAGATACT AGGCACCCCGAAGATGAGT IL1 β TGTGATGAAAGACGGCACACC GGGAACTGTGCAGACTCAAC IL6 CCCACCAGGAACGAAAGTCA TCTTGCGGAGAGAAACTT IL33 CCCGCCTTGCAAAATCACAA CCCTTCATGCTTGCTACCTGAT MPO CCACGGCCTTTCAATGTCAC TCTCGGTATGTGATGATCTGGA GDF15 TGACCCAGCTGTCCGGATAC GTGCACGCGGTAGGCTTC TAZ CGGGCAGAAAACAAGTCAGC AGCTGTTCTGCCTGCATCTT SOD TGGCTTGGCTTCAATAAGA AAGGTAGTAAGCGTGCTCC PLA2 ACCATCCCATCCAAGAGAGC CAAACTCCAGAAGGCTCCCC CDS2 ATTGGGGGCTTCTTTGCTAC TCAGATGGCTCACAGTCCAC CD68 CTCTCATCATTGGCCTGGTC GGGCTGGTAGGTTGATTGTC Mitochondrial isolation Fresh RV tissue, transported in 0.9% KCl, was minced in 5 ml of ice cold isolation medium A (220mM mannitol, 70 mM sucrose, 5 mM TES, 0.1 mM EGTA, pH 7.3) supplemented with proteinase (0.2 mg/ml) and left for 5 minutes. 20 ml of ice cold isolation medium A supplemented with bovine serum albumin (1mg/ml) and homogenize with Potter-Elvehjem homogenizer with 10 up-and-down at 750 rpm. Homogenate was centrifuged at 800 g for ten minutes at 4°C, where after the supernatant in a clean tube was centrifuged at 7200 g for ten minutes at 4°C. After discarding the supernant, the pellet was suspend in 2 ml ice cold medium A and