Anne-Marie Koop

234 Figure 1. Assessment of right ventricular hypertrophy induced by PAB upon cardiac overexpression of miR- 199b. a) Design of the in vivo study; b) Assessment of pulmonary artery banding gradient in wildtype (WT) and MHC-miR-199b Tg (MHC-199b) animals, by measuring RV pressure at 2 and 6 weeks after either sham or PAB surgery; c) Quantitative real-time PCR analysis of miR-199b expression in RV tissue from WT and MHC-199b animals either after sham or PAB surgery, n=5-8 hearts; d) Fulton’s index values (ratio of right ventricular weight to left ventricular plus septal weight) of WT and MHC-199b animals either after sham or PAB surgery, n=5-8 hearts; e) High-magnification of representative images of histological sections stained for haematoxylin & eosin (H&E), black scale bar is equivalent to 20 mm; f) High-magnification of representative images of histological sections stained for wheat germ agglutinin (WGA), black scale bar is equivalent to 20 mm; g) Quantification of cell surface areas in f, n=30 microscopic field/heart, 3 hearts; h) High-magnification of representative images of histological sections stained for Sirius-red, black scale bar is equivalent to 20 mm; i) Quantification of collagen deposition in h, n=30 microscopic field/heart, 3 hearts. Statistical analysis using One-way ANOVAwith Tukey’s multiple comparisons test. ∗ p<0.05 versus corresponding control group; #p<0.05 versus corresponding experimental group (error bars are s.e.m.).