Anne-Marie Koop

6 235 Figure 2. miR-199b overexpression impairs RV function during pressure overload-induced RV remodelling. Cardiac function was assessed in hearts from wildtype (WT) and MHC-miR-199b Tg (MHC-199b) animals, either after sham or PAB surgery, and followed by quantitative analysis. a) RV end-diastolic volumes (EDV) and b) RV end-systolic volumes, n=5-8 hearts; c) Representative MRI images of cardiac ventricular end- diastole and end-systole; d) Cardiac output, n=5-8 hearts; e) A Pearson’s correlation was run to determine the relationship between cardiac miR-199b expression levels and cardiac output in 22 mice, independent of the treatment and genotype. f) RV ejection fraction (EF), n=5-8 hearts; g) A Pearson’s correlation was run to determine the relationship between cardiac miR-199b expression levels and RV EF in 22 mice, independent of the treatment and genotype; h) echocardiographic tricuspid annular plane systolic excursion (TAPSE) measurements at the end of the study and i) every 2 weeks throughout the complete study, n=5-8 hearts. Statistical analysis using One-way ANOVA with Tukey’s multiple comparisons test. ∗ p<0.05 versus corresponding control group; #p<0.05 versus corresponding experimental group (error bars are s.e.m.).