Anne-Marie Koop

236 Figure 3 . RV expression profile of cardiac hypertrophy-related genes. Quantitative real-time PCR analysis was performed to assess the expression levels of several genes known to be related to cardiac hypertrophy in RV tissue from wildtype (WT) and MHC-miR-199b Tg (MHC-199b) animals, either after sham or PAB surgery. a) natriuretic peptides atrial natriuretic factor (Nppa); b) brain natriuretic peptide (Nppb); c) β -skeletal actin (Acta1); d) β -myosin heavy chain (Myh7); e) a-myosin heavy chain (Myh6); f) regulator of Calcineurin 1 Isoform 4 (Rcan1-4); g) nuclear NFAT kinase dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a); h) transforming growth factor beta (TGF- β ); i) endoglin; j) collagen type I alpha 1 chain (COL1A1); k) Capillaries in RV sections of wildtype (WT) and MHC-miR-199b Tg (MHC-199b) animals, either after sham or PAB surgery, were identified by isolectin B4 immunohistochemistry (yellow). WGA marks cell membranes (green), and Hoechst stain identifies