Anne-Marie Koop

258 <-1 and with parametric F-test comparing nested linear models (p value < 0.05) by R package Ballgown. 26 Statistical Analysis All data are presented as mean ± standard error of mean (SEM). The variables were analyzed using Student’s t-test and two-way analysis of variance (ANOVA) to assess statistical significance between groups (IBM SPSS Statistics for Macintosh, Version 25.0, released 2017; IBM Corp). The evaluation of significant effects evaluation was conducted using Tukey’s multiple comparison tests, with an adjusted calculation of p -value. Probability values p<0.05 were considered statistically significant.

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