Anne-Marie Koop

7 267 Figure 4 a 0 5 10 20 -10 -5 0 5 Hand2 F/F Ifi205 Tecrl AC022682.2 GM26956 Hnrnpa3 Vis1 Gpcpd1 Aldob Cenpf Slco1a4 Rbm6-ps2 Gm12096 Ltbp2 Mgat5b Nppa Mfap5 Mybpc2 Sprr1a Vaultrc5 Nppb Gm10566 Snord17 Rprl3 Gm26035 Abi3 Adam8 Gm26448 Unc5b Col4a1 Col4a2 Emilin1; Khk Adamtsl2 Gm26461 Acta1 Rcan1 Rnu3b2 Rnu3b4 Rnu3b1 Rmrp Scarna2 Lgals3 Cx3cl1 Tgfb3 Capg Bgn Timp1 Gpnmb Meox1 Chodl Loxl1 Tril Mmp14 Col5a3 Dpysl3 Col16a1 Adcy7 Col15a1 Mrc2 Faim2 Mgp Fmod Col14a1 Mfap4 Nbl1 Ssc5d Postn Cola52 Col1a1 Thbs4 Svep1 Fn1 Adamts2 Col3a1 Mmp2 Col1a2 Dact3 Col11a1 Fbn1 Col6a2 b Ahnak2 Scx Gm5869 proteinaceous extracellular matrix protein heterodimerization activity nucleosome neutrophil chemotaxis monocyte chemotaxis lymphocyte chemotaxis extracellular space extracellular region extracellular matrix structural constituent endodermal cell differentiation muscle cell differentiation collagen trimer collagen fibril organization circadian regulation of gene expression chemokine-mediated signaling pathway chemokine activity cell adhesion CCR7 chemokine receptor binding CCR chemokine receptor binding basement membrane rich factor c Rgs13 Zfp970; Gm14327 Pfkfb1 Aqp4 Fgf13 Col8a1 Ankrd23 Pmepa1 Comp Piezo2 Cilp Col8a2 Sfrp2 Pi16 Tnmd Fibin 0.2 0.4 0.6 15 25 10 Igfbp6 Col12a1 Hand2 F/F : PAB vs Sham log10 ( p value) log2 (fold change) sham PAB -1 -0.5 0 0.5 1 1.5 GO enrichment 0.8 Valine, leucine and isoleucine degradation Systemic lupus erythematosus Rheumatoid arthritis Protein digestion and absorption Platelet activation PI3K-Akt signaling pathway Focal adhesion Malaria Hypertrophic cardiomyopathy (HCM) Propanoate metabolism ECM-receptor interaction Dilated cardiomyopathy (DCM) Cytokine-cytokine receptor interaction Chemokine signaling pathway cGMP-PKG signaling pathway Arrhythmogenic right ventricular cardiomyopathy Amoebiasis Alcoholism AGE-RAGE signaling pathway in diabetic complications TNF signaling pathway 0.15 0.2 0.25 rich factor Pathway enrichment 6e-06 3e-06 9e-06 80 120 160 0e+00 40 p value nrofgenes 0.02 0.01 0.03 20 30 10 p value nrofgenes d Figure 4 . Transcriptional changes in the pressure-overloaded RV. RNA-sequencing was performed to assess the transcriptomic changes in RV tissue from control (Hand2F/F) animals subjected to either sham or PAB surgery for 6 weeks. a) Volcano plot representation of differential expression of genes in the RV of sham and