Anne-Marie Koop

290 Table 2. Levels of biomarkers per type of RV overload All patients Pressure overload Volume overload Biomarkers n Median (IQR) n Median (IQR) n Median (IQR) hs TnT, ng/l 124 5.0 (4.0-7.0) 55 6.0 (4.0-7.0) 25 5.0 (4.0-6.0) NT-proBNP, ng/l 124 113 (62-209) 55 143 (75-251) 25 86 (56-179) Galectin-3, ng/ml 125 12.4 (10.6-15.5) 56 12.8 (10.2-16.6) 25 11.9 (10.2-14.8) MR-proADM, nmol/l 125 0.389 (0.340-0.451) 56 0.398 (0.344-0.508) 25 0.374 (0.340-0.436) GDF-15, pg/ml 124 352 (281-454) 55 384 (299-524) 25 349 (263-452) MPO, ng/ml 125 27.3 (19.2-41.5) 56 31.1 (22.2-49.0) 25 25.3 (18.2-35.5) ET-1, pg/ml 124 1.46 (1.19-1.95) 55 1.67 (1.36-2.74) 25 1.32 (1.10-1.79) Neprilysin, ng/ml 124 0.81 (0.00-6.38) 55 0.69 (0.03-10.07) 25 1.20 (0.22-14.54) hs TnT: high-sensitive Troponin T; NT-proBNP: N-terminal pro-B-Type natriuretic peptide; ET-1: Endothelin-1; MR-proADM: midregional proadrenomedullin; MPO: myeloperoxidase; GDF-15: growth differentiation factor 15. * significant difference between no residual overload and pressure overload. † significant difference between combined overload and pressure overload. ‡ p-value from Kruskal Wallis test Figure 1. Patterns of changes in plasma levels in the combined panel of eight biomarkers according to type and degree of RV overload compared with no residual RV overload. NT-proBNP: N-Terminal pro-B- Type natriuretic peptide; MR-proADM: midregional proadrenomedullin; Gal-3: galectin-3; GDF-15: growth differentiation factor 15; MPO: myeloperoxidase; ET-1: endothelin-1; hsTnT: high sensitive Troponin T; NP: neprilysin. 0 1 2 3 4 5 hsTnT NT-proBNP Gal-3 MR-proADM GDF-15 MPO ET-1 NP pressure overload No residual loading Mild-moderate loading Severe loading 0 1 2 3 4 5 hsTnT NT-proBNP Gal-3 MR-proADM GDF-15 MPO ET-1 NP volume overload no residual load mild-moderate severe overload p-value mild-moderate severe overload p-value (n=16) overload (n=29) (n=27) overload (n=15) (n=12) hsTnT 4.0 (0.8-5.0) 4.0 (3.0-6.0) 6.0 (5.0-8.0) 0.001*† 5.0 (4.0-6.0) 5.0 (4.0-9.5) 0.228 NT-proBNP 48 (33-128) 106 (63-149) 229 (112-314) <0.001*† 85 (57-162) 126 (48-357) 0.164 Gal-3 11.8 (9.6-13.5) 12.0 (10.2-15.9) 13.6 (10.0-16.8) 0.193 12.5 (9.7-14.9) 12.1 (10.4-16.2) 0.728 MR-proADM 0.395 (0.324-0.427) 0.388 (0.325-0.492) 0.439 (0.363-0.524) 0.147 0.368 (0.339-0.444) 0.368 (0.342-0.412) 0.924 GDF-15 284 (250-392) 391 (276-607) 364 (329-495) 0.021* 333 (285-433) 376 (251-458) 0.333 MPO 23.2 (17.1-29.4) 29.9 (21.4-42.1) 31.2 (23.4-61.5) 0.078 27.3 (19.9-35.3) 24.8 (17.0-49.2) 0.834 ET-1 1.17 (0.85-1.45) 1.48 (1.33-1.89) 2.39 (1.48-3.05) <0.001*‡ 1.29 (1.09-1.86) 1.34 (1.13-1.81) 0.15 NP 1.38 (0.00-7.24) 0.74 (0.13-11.63) 0.62 (0.00-10.07) 0.900 0.53 (0.16-22.78) 1.25 (0.02-10.41) 0.965