Anne-Marie Koop

74 3 ). Unfortunately, protein levels of HK1 were only determined in one study 18 and HK2 protein levels were not determined at all, and therefore it is unclear how HK protein levels are affected by pressure overload. Glycolysis was studied on isolated hearts in a Langendorf perfusion system, of three RV pressure overload models: MCT 15 , PAB 21 and FHR 16 . In addition, glycolysis was determined by Seahorse in RV preparations of the FHR-model 16 . Meta-analysis of the data revealed that glycolysis was significantly increased in cardiac tissue of these RV pressure loaded hearts ( figure 3d ). Right ventricular glucose uptake and glycolysis are increased in conditions of increased pressure load.