Anne-Marie Koop

76 Figure 3. Glycolysis. Forrest plot of HK1 (A) and bubble plot showing meta-regression analysis of HK1 expression at mRNA level with the duration of RV pressure load (B). Forrest plots of HK2 (C) expression at mRNA level and glycolytic flux measured with Seahorse or Langendorf (D). Data are presented as Hedges’ g. Combined Hedges’ g are presented as squares: grey representing Hedges’ g of a specific model, black representing Hedges’ g of all included studies. Bars represent 95% confidence interval. Bubble size represents relative study precision, calculation based on standard deviation. Black line represents regression line, grey lines represents 95% confidence interval. SuHx = Sugen hypoxia, PAB = pulmonary artery banding, MCT = monocrotaline, FHR = fawn hooded rats, PH = pulmonary hypertension, HK = hexokinase. CO = cardiac output, CI = cardiac index, TAPSE = tricuspid annular plane systolic movement, ↓ = decreased, “=” = not statistically significant affected. 95% CI = 95% confidence interval, n = number of included animals, i² = level of heterogeneity, X = not included in meta-analysis. Transport of fatty acids Transporter cluster differentiation 36 (CD36), the main transporter of fatty acids across the plasma membrane, was only investigated in three studies (either RNA or protein) 27,37,41 and hence did not meet the criteria for meta-analysis. Transport of fatty acids over the mitochondrial membrane is highly regulated by carnitine palmitoyltransferases (CPT1 and CPT2 ) (outer and inner membrane, respectively). Only meta-analysis of subunit CPT1B was possible, but revealed ambivalent and non-significant results 16,27,34,37 ( figure 4a ). However, CPT1B mRNA negatively correlated with duration of pressure overload ( figure 4b ).