Sara van den Berg

111 4 CMV on influenza infection Supplementary Figure 1. Percentage of CD8+ T-cells per t-SNE cluster in CMV- and CMV+ individuals. Percentage of CD8 + T-cells per t-SNE cluster in CMV- (blue bars; N=40) and CMV+ (red bars; N=57) individuals based on MFI of CD57, KLRG-1, CXCR3, CD95, CD127, CD45RO, CD27 and CCR7. Data shown as median. Differ- ences between groups were compared by Mann Whitney. Stars indicate statistical differences. Supplementary Figure 2. Influenza virus-specific IFN γ T-cell response in CMV- and CMV+ individuals differentiated on the level of CMV-specific antibodies. A) All influenza virus-specific IFNy response at <72 hours, 2 weeks and 8 weeks after fever onset, depicted as percentage of total CD3 T-cells (left panel), and com- pared between CMV- and CMV+ individuals (right panel). B) Correlation between CMV-specific antibody levels and influenza virus-specific T-cell responses in CMV+ individuals on the three time points after fever onset. Influenza-specific IFN γ T-cell responses are depicted in blue open circles in scatter plot for CMV- individuals and in red solid circles in correlation of CMV-specific IFN γ T-cell responses for CMV+ individuals. Correlation was assessed by Spearman correlation.