Sara van den Berg

112 Chapter 4 Supplementary Figure 3. Expression of activation and migration markers in T-cells during influenza virus infection. A) The percentage of CD127-KLRG-1+ and HLA-DR+CD38+ influenza virus-specific CD8 + T-cells upon influenza infection in all HLA-A2 positive individuals upon influenza infection (left panels), or comparing CMV-(blue bars) and CMV+ (red bars) (middle panels). Right panels shows CD127-KLRG-1+ and HLA-DR+CD38+ expression on CMV-specific CD8 + T-cells. Influenza virus-specific CD8+ T-cells were identified using a tetramer for matrix protein-1 GILG-epitope. CMV-specific CD8+ T-cells were identified using a tetramer for pp65 protein NLV-epitope. B) Frequencies of CD3 + T-cells in the blood of influenza virus-infected individuals at <72 hours, 2 weeks and 8 weeks after infection (left panel) and in CMV- and CMV+ individuals (right panel). C) The change in percentage of CD3 + T-cells at time point 1 compared to time point 2 in CMV- and CMV+ individuals. D) Percentage of CXCR3+ within total CD8 + T-cells upon infection (left panel) and in CMV- and CMV+ individuals (right panel). E) CXCR3+ influenza-virus specific CD8+ T-cells upon infection (left panel) and CMV- and CMV+ individuals (middle panel) and on CMV-specific CD8+ T-cells (right panel). Wilcoxon test was used to compare time points. Differences between CMV- and CMV+ individuals were tested using Mann-Whitney U test.