Sara van den Berg

113 4 CMV on influenza infection Supplementary Figure 4. Serum levels of IFN γ and IL-10 in CMV- and CMV+ individuals after influenza virus-infection. Serum levels of IFN γ and IL-10 upon influenza infection for CMV+ and CMV- individuals at <72hours after fever onset, and 2 and 8 weeks later. CMV- individuals are indicated with the blue open circles and the CMV+ individuals in red solid circles. Serum levels of the cytokines were measured by multiplex assays and batch effects were normalized based by subtracting the mean per plate. Differences between CMV- and CMV+ individuals were tested using unpaired T-test. Differences between time points were tested by Wilcoxon test. Supplementary Figure 5. Duration of symptoms is not linked to T-cell responses to influenza virus-infec- tion. A) Association was tested for influenza-specific T-cell responses upon influenza virus infection at <72 hours after fever onset, and 2 and 8 weeks later with the duration of symptoms of influenza infection. B) Association was tested for influenza-specific T-cell responses upon influenza virus infection at <72 hours after fever onset, and 2 and 8 weeks later with the number of symptoms. Due to study design, participants had a minimal of two symptoms; fever (≥37.8 °C) and at least 1 other symptoms, either cough, sore throat, runny nose, headache, pain while breathing or muscle pain. Associations was tested by Spearman correlation.