Sara van den Berg

153 6 T-cell dynamics in CMV Figure 4. Dynamics of CMV-specific CD8 + T-cells and CD8 + T EM/EMRA cells in CMV+ and CMV- individuals. A. Frequency within the total CD8 + T-cell pool (upper panel) and absolute count (lower panel) of CMV-specific CD8 + T-cells over time in the CMV+ individuals selected for the deuterium labelling study. B. Deuterium labelling enrichment (%DNA labelled) of CMV-specific CD8 + T-cells compared to CD8 + T EM/EMRA cells of CMV+ (upper panel) or CMV- (lower panel) individuals. Label enrichment was scaled between 0 and 100% by normalizing for the estimated maximum enrichment of granulocytes (see Materials and methods ). Lines go through the mean of duplicate measurements. It should be noted that for one individual (E34) it was not possible to determine the pro- duction rate of CD8 + T EM/EMRA cells because too many samples were out of range of the standard lines for deuterium enrichment measurement. C. Estimated average production rates of CMV-specific CD8 + T-cells compared to CD8 + T EM/EMRA cells of CMV+ (upper panel) or CMV- (lower panel) individuals. All estimates were obtained by fitting a single-exponential model to the data sets per individual (see Materials and methods and Supplementary Figure 5 ). D. Comparison of production and loss rate of CMV-specific T-cells. E. Association between the absolute cell number and the average production rate of CMV-specific CD8 + T-cells. Horizontal lines represent median values.