Sara van den Berg

164 Chapter 6 Supplementary Figure 2. Percentage of CD8 + T-cells per cluster in the t-SNE analysis. CD8 + T-cells of CMV- and CMV+ individuals were clustered by t-SNE analysis based on the expression of CD57, KLRG-1, CD127, CD27, CD45RO, CCR7, and CD95 (see Figure 1E ). Nine clusters were identified within the t-SNE plot were identified based on cell density. Here the percentage of CD8 + T-cells that fall into each of these clusters out of total CD8 + T-cells is shown for CMV- and CMV+ individuals. Differences between CMV- and CMV+ individuals were tested by Mann-Whitney U test. Stars indicate significant differences as follows: * P -value <0.05, ** P -value <0.01, *** P -value <0.001, *** P -value <0.0001.