Sara van den Berg

48 Chapter 2 Influenza antibody titer Protection Parameter B (beta) Standard Error Sig (P-value) B (beta) Standard Error Sig (P-value) (Intercept) 3.895 0.7973 0 -1.777 0.861 0.039 Age group 2 (40-52 year) -0.347 0.7905 0.661 -0.428 0.817 0.601 Age group 1 (30-40 year) -0.519 0.8256 0.53 -0.373 0.8499 0.66 Age group 0 (18-30 year) 0 a . . 0 a . . Sex male -0.093 0.3691 0.801 0.475 0.4621 0.304 Sex female 0 a . . 0 a . . Previous influenza vaccinations yes 0.407 0.5556 0.464 0.81 0.6561 0.217 Previous influenza vaccinations sometimes 0.407 0.4929 0.409 1.406 0.6414 0.028 Previous influenza vaccinations no 0 a . . 0 a . . Seasonal 2009 vaccination yes 0.566 0.6298 0.368 0.763 0.6701 0.255 Seasonal 2009 vaccination no 0 a . . 0 a . . CMV IgG group high 0.674 0.3982 0.09 1.033 0.6058 0.088 CMV IgG group medium 0.35 0.5267 0.506 0.836 0.6501 0.199 CMV IgG group low 0 a . . 0 a . . Timepoint 3 1.003 0.2659 < 0.001 0.989 0.3764 0.009 Timepoint 2 2.043 0.3249 < 0.001 1.965 0.5822 0.001 Timepoint 1 0 a . . 0 a . . CMV IgG group high * Timepoint 3 0.554 0.4295 0.197 1.211 1.0022 0.227 CMV IgG group high * Timepoint 2 0.598 0.5711 0.295 0.741 1.1863 0.532 CMV IgG group high * Timepoint 1 0 a . . 0 a . . CMV IgG group medium * Timepoint 3 0.387 0.5298 0.465 0.31 0.7386 0.675 CMV IgG group medium * Timepoint 2 -0.043 0.6415 0.946 -0.339 0.8217 0.68 CMV IgG group medium * Timepoint 1 0 a . . 0 a . . CMV IgG group low * Timepoint 3 0 a . . 0 a . . CMV IgG group low * Timepoint 2 0 a . . 0 a . . CMV IgG group low * Timepoint 1 0 a . . 0 a . . Supplementary Table 7. Regression table effect CMV IgG group serostatus on seasonal influenza vaccine response of H3N2 strain in the season 2010-2011. Bold: p value < 0.10 Bold and underlined: p value < 0.05 a reference category Time point Correlation age and influenza titer Differences between age groups in influenza titer T2 p=0.0013, R -0.198 p=0.016 T3 p=0.000, R= -0.269 p=0.000 T4 p=0.006, R= -0.172 p=0.015 T5 p=0.165, R=-0.112 p=0.714 Supplementary Table 8. Negative effect of age on titers after de novo pandemic influenza vaccination . Correlations between age and H1N1pdm influenza antibody titer is presented on all time points of the pandemic season (Pearson correlation). Differences H1N1pdm influenza antibody titers after vaccination (T2-T5) (log transformed) between age groups (18-30 year, 30-40 year, 40-52 year) are presented (ANOVA). P < 0.05 is considered significant.