Milea Timbergen

106 Data Availability Statement The datasets generated for this study can be found in the Sequence Read Archive (SRA) data repository under accession number PRJNA604749. Conflict of Interest The authors declare no conflict of interest or financial interests except for R.B., J.B., W.V.I. and J.G., who report being shareholder in Methylomics B.V., a commercial company that applies MeD-seq to develop methylation markers for cancer staging. Funding This research received no external funding. Acknowledgments We thank G.J.L.H. van Leenders (Department of Pathology, Erasmus MC) for his assistance with the selection and pathological assessment of the DTF tumour tissues. 4