Milea Timbergen

118 Supplemental Figure 5. DNMT1 is not co-precipitated with wild-type or mutant CTNNB1 (β-catenin). HCT116 cells were transfected with plasmids driving the expression of FLAG-tagged wild-type β-catenin (WT) or FLAG-tagged mutant versions of β-catenin (T41A; S45P; Exon 3 deletion mutant; K335I). As a control cells were transfected with the empty vector. At 48 h post-transfection cell lysates were prepared from which the FLAG-tagged β-catenin variants were immunoprecipitated. Western Blot analysis was used to examine DNMT1, β-catenin and β-actin protein expression in the total lysates and immunoprecipitates. 4