Milea Timbergen

65 Figure 1. Dot plot depicting the individual data relative expression levels measured with realtime qPCR of Wnt target genes AXIN2, DKK1 and CCND1 in DTF harbouring either a S45F or T41A CTNNB1 mutation or wild-type DTF. Next, mRNA expression data from the French cohort were used to validate these findings. The patient and tumour characteristics of this cohort are listed in Table 1. Ninety-three desmoid samples; CTNNB1 WT (n = 14); T41A (n = 45) and S45F (n = 34) were selected for subsequent analysis. First, the Affymetrix probe sets for AXIN2 (222696_at, 224176_at, 222695_s_at, 224498_x_at ) CCND1 (208712_at, 208711_s_at) and DKK1 (204602_at) were identified and the probes that displayed the most variable expression values after log 2 transformation, based on the highest IQR, were selected for further analysis ( 224498_x_at , 208711_s_at and 204602_at). A Kruskal-Wallis test showed no significantly differences between the mean ranks of the groups with T41A or S45F mutations and WT CTNNB1 (Table 2). 3