Els van de Vijver

59 Supplementary file 4: Detailed description of discordant pairs of calprotectin and calgranulin-C results (n=31). Attending paediatrians were blinded to the viral PCR result, which explains the discrepancy in diagnosis in the lowest five patients. DISCORDANT RESULTS LOW CALPROTECTIN - HIGH CALGRANULIN-C Calprotectin (µg/g) Calgranulin-C (µg/g) Bacterial and parasite PCR IBD risk stratum Diagnosis Post-hoc viral PCR 315 1.22 negative High CD negative 340 1.03 D. fragilis High CD negative 150 1.06 Giardia lamblia High GI-infection negative 165 3.17 D. fragilis High GI-infection negative 105 1.98 negative High IBS negative 220 1.11 negative High IBS negative 255 1.82 D. fragilis Low IBS negative 40 8.04 negative Low HUS negative 345 1.62 D. fragilis High solitary rectal ulcer negative DISCORDANT RESULTS HIGH CALPROTECTIN - LOW CALGRANULIN-C Calprotectin (µg/g) Calgranulin-C (µg/g) Bacterial and parasite PCR IBD risk stratum Diagnosis Post hoc viral PCR 480 0.42 D. fragilis High UC negative 510 0.23 STEC High CD negative 855 0.49 negative High CD negative 980 0.24 negative High CD negative 1170 0.54 negative High UC negative 1270 0.33 negative High CD negative 1280 0.22 negative High CD negative 1410 0.22 D. fragilis High UC negative 1440 0.22 negative High CD negative 1660 0.26 negative High CD negative 2080 0.68 negative High CD negative 645 0.22 STEC High GI-infection negative 1120 0.53 negative High spontaneous recovery negative 1370 0.24 Giardia lamblia Low GI-infection negative 1380 0.22 STEC Low GI-infection negative 2550 0.22 negative Low GI-infection negative 580 0.22 negative Low allergy negative 585 0.58 STEC, D. fragilis, High IBS parechovirus 1160 0.22 D. fragilis High IBS adenovirus 685 0.22 negative Low reflux oesophagitis adenovirus 1150 0.22 negative High spontaneous recovery norovirus 2510 0.24 D. fragilis Low spontaneous recovery Norovirus CD, Crohn;s disease; GI-infection, gastrointestinal infection; HUS, haemolytic uraemic syndrome; IBS, irritable bowel syndrome; STEC, shiga toxin-producing E. coli; UC, ulcerative colitis Head-to-head comparison Calgranulin C and Calprotectin 59