Adriëtte Oostvogels

3 79 pBMI, lipids and offspring’s body composition Maternal lipid measurements and pBMI Non-fasting blood sampling was conducted. For each woman a blood sample was taken in a 9-mL Vacuette (Greiner BV, Alphen aan de Rijn, the Netherlands) for the preparation of serum. From this sample triglycerides TG, total cholesterol (TC), apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB) and free fatty acids (FFA) were determined as follows. TG was tested with the glycerol-3-phospate oxidase PAP method. TC was assessed using the cholesterol oxidase PAP method on a Hitachi 912 analyser (Roche Diagnostics, Mannheim, Germany). The interassay coefficient of variation was 2.3% for TG and 1.0% for TC. ApoA1, ApoB and FFA analyses were done with an Abbot Architect CI 8200 (Abbott Laboratories, Limited. Saint-Laurent, Québec, Canada). A turbidimetric technique was used for analysing ApoA1 and ApoB. The FFA analyses were done with a combination of enzymatic and spectrophotometry/ colorimetry (reagent by Wako Pure Chemical Industries Ltd. (Osaka, Japan)). The interassay coefficient of variation was 1.6% for ApoA1, 1.6% for ApoB and 3.4% for FFA. Figure 1. Flowchart of the study population. * Numbers sum to more than 229 as more than one exclusion criterion per mother was sometimes present.