Jos Jansen

46 Chapter 2 Figure 4. Immunofluorescence staining and localization of CCDC115-V5 in HeLa cells . HeLa cells were transiently transfected with a V5-tagged CCDC115 construct and then fixed and stained with immunofluorescently labeled antibodies against V5 (green in merge) and different organelle markers (magenta in merge). Shown are representative cells stained for CCDC1155 organelle markers, and a merge with DAPI stain (blue), including a 3-fold magnification. Co-localization is indicated by white color in the merged channel. The graphs show the fluorescence intensity profiles along the crosssections indicated. Scalebars represent 5 μ m.