Jos Jansen

2 57 CCDC115 Deficiency Causes a Disorder of Golgi Homeostasis with Abnormal Protein Glycosylation Table S1. Primer overview Forward 5’ 3’ Reverse 5’ 3’ Primers for Sanger sequencing CCDC115 exon 1 CCACTCTTCCCTCAACCAAA TCCATGTGGGAAGCATACTG CCDC115 exon 2 AGAAGGAAGAGGGGGCTCAC CAGATCTGGAGGTGTGTCCA CCDC115 exon 3 CCCAGTGTAAAGCAACTCACC TCTCACCGCTTTCCTCTCC CCDC115 exon 4 TTTGGATGGAGGAAAACCTG GTTTCCTGGAACCCCAAAGT CCDC115 exon 5 GGCTGAGTACGGTTTTCTCC CCTCAGGGTTCTGGAGCAG M13 tag tgtaaaacgacggccagt caggaaacagctatgacc Primers for cloning of the pcDNA3.1- CCDC115 -V5 construct CCDC115 cDNA GGCAGCCCCAGGCTCCAG ATGGCGGCGCTTGACCTGC Primers for MLPA CCDC115 5’ Exon 1 gggttccctaagggttggaACCAACAG- GAGAACCCAATGGGCAAGGAA AACGGCCGGCAAGCATTTGGAAAGAC- GCTtctagattggatcttgctggcac CCDC115 Exon 1-2 gggttccctaagggttggaAGGAGGGTACACG- GGCGTAAACTGA GTCTCACCGCTTTCCTCTCCCTG- CAtctagattggatcttgctggcac CCDC115 Exon 3 gggttccctaagggttggaTTCTCGTCCTTCAG- CGAGGCCCAGGA GGGACTCCAGAAGTTCAAGGTGGT- GAggcgtatagggtgccgtggttgtctagattg- gatcttgctggcac CCDC115 Exon 4 gggttccctaagggttggaCAGGCTCAAG- CAAGCTTCCGGGATGGTGAGTG GACCGTGTTGTTTGGCTCTGTGGC- CCTCAGACgggcgtatagggtgccgtggttgta- atctagattggatcttgctggcac CCDC115 Exon 5 gggttccctaagggttggaATCCAGATCCTCCT- GGGTCGTGTGTTACACTAC AGATACCATGGT- GACCTTCTCTCCTTTCCTCCAtctagattg- gatcttgctggcac SMPD4 Exon 1 gggttccctaagggttggaGCCTTCGCTTTGC- GCACGCGCCTTTTGAGGT AACGGCCCAAAGAGGTGGAAGC- GCTTTTCCCggcgtatagggtgccgtg- gttctagattggatcttgctggcac PTPN18 Exon 2-3 gggttccctaagggttggaTAGCTGAAAGC- CAGCAGGGGACCACACACATGC AGAGATCCCACCAAGGTGCCCTTGTG- GGCTGCTgtgggcgtatagggtgccgtggttgta- agtggtctagattggatcttgctggcac