Sanne Hoeks

TH17 within first three months of life 49 4 Fig 1 FIGURE 1. Until age 12 months, most TH cells remain naive and most FOXP3 + cells are resting Treg cells found in population I. After thawing, PBMCs were stained for CD3, CD4, CD45RA, CD45RO, and intracellularly for FOXP3. A, Representative plots for CD45RA + /CD45RO + CD4 + T-cell distribution and fraction of CD45RA + CD45RO- and CD45RA-CD45RO + CD4 + T cells. B, Distribution of Treg-cell populations according to Miyara et al 4 for cells derived at different ages. C, IL-1β, D, IL-6, and E, TNFα concentration in the supernatant after 4 hours incubation of PBMCs with phorbol 12-myristate 13-acetate and ionomycin. Data as mean ± SEM. *P < .05, **P < .01, ***P < .001, compared with adult.